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chk 166 n297q  (Bio-Rad)


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    Structured Review

    Bio-Rad chk 166 n297q
    Figure 3. Virus accumulation in the medullary sinus and fluid phase of the DLN is reduced after anti-GR-1 treatment (Four-week-old male C57BL/6J mice pre-treated (day 1) with anti-GR-1 or isotype control antibody were inoculated in the footpad with 103 FFU of CHIKV-LR. (A) DLNs were harvested, and lymphatic fluid was collected. After clarification by centrifugation, a plaque assay was used to quantify infectious virus (n = 6–7, two experiments). (B and C) DLNs were harvested, and tissue homogenates were assessed for infectious virus by plaque assay (B) and viral RNA (C) (n = 6–7, two experiments). (D) Popliteal DLNs were harvested at 18 hpi, fixed, incubated in 30% sucrose for 48 h, cryo-sectioned, and then stained with the following anti- bodies: anti-CHIKV E1 (CHK-166 <t>N297Q,</t> white), anti-ERTR7 (red), and anti-CD169 (green). Sections were imaged using confocal microscopy and representative
    Chk 166 N297q, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chk+166+n297q/Rat+anti+Mouse+ER-TR7/pm38446669-260-20-26
    Average 92 stars, based on 29 article reviews
    chk 166 n297q - by Bioz Stars, 2026-09
    92/100 stars

    Images

    1) Product Images from "Ly6C + monocytes in the skin promote systemic alphavirus dissemination."

    Article Title: Ly6C + monocytes in the skin promote systemic alphavirus dissemination.

    Journal: Cell reports

    doi: 10.1016/j.celrep.2024.113876

    Figure 3. Virus accumulation in the medullary sinus and fluid phase of the DLN is reduced after anti-GR-1 treatment (Four-week-old male C57BL/6J mice pre-treated (day 1) with anti-GR-1 or isotype control antibody were inoculated in the footpad with 103 FFU of CHIKV-LR. (A) DLNs were harvested, and lymphatic fluid was collected. After clarification by centrifugation, a plaque assay was used to quantify infectious virus (n = 6–7, two experiments). (B and C) DLNs were harvested, and tissue homogenates were assessed for infectious virus by plaque assay (B) and viral RNA (C) (n = 6–7, two experiments). (D) Popliteal DLNs were harvested at 18 hpi, fixed, incubated in 30% sucrose for 48 h, cryo-sectioned, and then stained with the following anti- bodies: anti-CHIKV E1 (CHK-166 N297Q, white), anti-ERTR7 (red), and anti-CD169 (green). Sections were imaged using confocal microscopy and representative
    Figure Legend Snippet: Figure 3. Virus accumulation in the medullary sinus and fluid phase of the DLN is reduced after anti-GR-1 treatment (Four-week-old male C57BL/6J mice pre-treated (day 1) with anti-GR-1 or isotype control antibody were inoculated in the footpad with 103 FFU of CHIKV-LR. (A) DLNs were harvested, and lymphatic fluid was collected. After clarification by centrifugation, a plaque assay was used to quantify infectious virus (n = 6–7, two experiments). (B and C) DLNs were harvested, and tissue homogenates were assessed for infectious virus by plaque assay (B) and viral RNA (C) (n = 6–7, two experiments). (D) Popliteal DLNs were harvested at 18 hpi, fixed, incubated in 30% sucrose for 48 h, cryo-sectioned, and then stained with the following anti- bodies: anti-CHIKV E1 (CHK-166 N297Q, white), anti-ERTR7 (red), and anti-CD169 (green). Sections were imaged using confocal microscopy and representative

    Techniques Used: Virus, Control, Centrifugation, Plaque Assay, Incubation, Staining, Confocal Microscopy

    Related Articles

    Staining:

    Article Title: Ly6C + monocytes in the skin promote systemic alphavirus dissemination.
    Article Snippet: .. Frozen sections (16 mm thick) were blocked with 5% donkey and bovine serum and then stained with the following Abs: CHK-166 N297Q (anti-E1),55–57 ERTR7 (clone ER-TR7, BioRad, Cat # MCA2402), and CD169 (clone 3D6.112, Biolegend, Cat # 142406). .. Sections were incubated with anti-human secondary antibody (1:200 polyclonal, Jackson ImmunoResearch, Cat# 109-035-003), and images were acquired on a Stellaris confocal microscope with TauSeparation enabled using identical PMT (photomutiplier tube) and laser settings between samples.



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    chk 166 n297q  (Bio-Rad)
    92
    Bio-Rad chk 166 n297q
    Figure 3. Virus accumulation in the medullary sinus and fluid phase of the DLN is reduced after anti-GR-1 treatment (Four-week-old male C57BL/6J mice pre-treated (day 1) with anti-GR-1 or isotype control antibody were inoculated in the footpad with 103 FFU of CHIKV-LR. (A) DLNs were harvested, and lymphatic fluid was collected. After clarification by centrifugation, a plaque assay was used to quantify infectious virus (n = 6–7, two experiments). (B and C) DLNs were harvested, and tissue homogenates were assessed for infectious virus by plaque assay (B) and viral RNA (C) (n = 6–7, two experiments). (D) Popliteal DLNs were harvested at 18 hpi, fixed, incubated in 30% sucrose for 48 h, cryo-sectioned, and then stained with the following anti- bodies: anti-CHIKV E1 (CHK-166 <t>N297Q,</t> white), anti-ERTR7 (red), and anti-CD169 (green). Sections were imaged using confocal microscopy and representative
    Chk 166 N297q, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chk+166+n297q/Rat+anti+Mouse+ER-TR7/pm38446669-260-20-26
    Average 92 stars, based on 1 article reviews
    chk 166 n297q - by Bioz Stars, 2026-09
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 3. Virus accumulation in the medullary sinus and fluid phase of the DLN is reduced after anti-GR-1 treatment (Four-week-old male C57BL/6J mice pre-treated (day 1) with anti-GR-1 or isotype control antibody were inoculated in the footpad with 103 FFU of CHIKV-LR. (A) DLNs were harvested, and lymphatic fluid was collected. After clarification by centrifugation, a plaque assay was used to quantify infectious virus (n = 6–7, two experiments). (B and C) DLNs were harvested, and tissue homogenates were assessed for infectious virus by plaque assay (B) and viral RNA (C) (n = 6–7, two experiments). (D) Popliteal DLNs were harvested at 18 hpi, fixed, incubated in 30% sucrose for 48 h, cryo-sectioned, and then stained with the following anti- bodies: anti-CHIKV E1 (CHK-166 N297Q, white), anti-ERTR7 (red), and anti-CD169 (green). Sections were imaged using confocal microscopy and representative

    Journal: Cell reports

    Article Title: Ly6C + monocytes in the skin promote systemic alphavirus dissemination.

    doi: 10.1016/j.celrep.2024.113876

    Figure Lengend Snippet: Figure 3. Virus accumulation in the medullary sinus and fluid phase of the DLN is reduced after anti-GR-1 treatment (Four-week-old male C57BL/6J mice pre-treated (day 1) with anti-GR-1 or isotype control antibody were inoculated in the footpad with 103 FFU of CHIKV-LR. (A) DLNs were harvested, and lymphatic fluid was collected. After clarification by centrifugation, a plaque assay was used to quantify infectious virus (n = 6–7, two experiments). (B and C) DLNs were harvested, and tissue homogenates were assessed for infectious virus by plaque assay (B) and viral RNA (C) (n = 6–7, two experiments). (D) Popliteal DLNs were harvested at 18 hpi, fixed, incubated in 30% sucrose for 48 h, cryo-sectioned, and then stained with the following anti- bodies: anti-CHIKV E1 (CHK-166 N297Q, white), anti-ERTR7 (red), and anti-CD169 (green). Sections were imaged using confocal microscopy and representative

    Article Snippet: Frozen sections (16 mm thick) were blocked with 5% donkey and bovine serum and then stained with the following Abs: CHK-166 N297Q (anti-E1),55–57 ERTR7 (clone ER-TR7, BioRad, Cat # MCA2402), and CD169 (clone 3D6.112, Biolegend, Cat # 142406).

    Techniques: Virus, Control, Centrifugation, Plaque Assay, Incubation, Staining, Confocal Microscopy